The effects of 'cell age' upon the lethal effects of physical and chemical mutagens in the yeast, Saccharomyces cerevisiae
Creators
- 1. University Coll. of Swansea (UK). Dept. of Genetics
- 2. Koeln Univ. (F.R. Germany). Inst. fuer Genetik
- 3. Welsh National School of Medicine, Cardiff (UK)
Description
Yeast cultures progressing from the exponential to the stationary phase of growth showed changes in cell sensitivity to physical agents such as UV light, heat shock at 520C and the chemical mutagens ethyl methane sulphonate, nitrous acid and mitomycin C. Exponential phase cells showed maximum resistance to heat shock and the three chemicals. The increased resistance of exponential phase cells to UV light was shown to be dependent upon the functional integrity of the RAD50 gene. Treatment of growing yeast cultures with radioactively labelled ethyl methane sulphonate indicated the preferential uptake of radioactivity during the sensitive exponential stage of growth. The results indicated that the differential uptake of the chemical mutagens was responsible for at least a fraction of the variations in cell sensitivity observed in yeast cultures at different phases of growth. (orig.)
Abstract (German)
Hefekulturen, die von der exponentiellen zur stationaeren Wachstumsphase uebergingen, zeigten Aenderungen in der Empfindlickeit gegenueber physikalischen Agentien wie z. B. UV-Strahlung und Erhitzung auf 520C sowie chemischen Mutagenen wie Aethylmethansulfonat, salpetriger Saeure und Mitomycin C. Zellen im exponentiellen Wachstum zeigten eine maximale Resistenz gegen UV-Licht und eine minimale gegenueber Hitzeschock und den drei Mutagenen. Die Zunahme der UV-Resistenz ist abhaengig von der funktionellen Unversehrtheit des RAD50-Gens. Eine Behandlung der Hefekulturen mit radioaktiv markiertem Aethylmethansulfonat zeigte, daβ die Aufnahme bevorzugt im exponentiellen Stadium stattfindet. Die Ergebnisse zeigen, dass die unterschiedliche Aufnahme der chemischen Mutagene wenigstens fuer einen Teil der Aenderung der Empfindlichkeit verantwortlich sind, die bei Hefekulturen zu verschiedenen Wachstumsphasen auftreten. (orig./MG)Additional details
Identifiers
- DOI
- 10.1007/bf00267979;
Publishing Information
- Journal Title
- Molecular and General Genetics MGG
- Journal Volume
- 146
- Journal Issue
- 1
- Series
- Mol. Gen. Genet.
- Journal Page Range
- 27-35
- ISSN
- 0026-8925
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 7270614
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- EMS; GROWTH; HEAT TREATMENTS; IRRADIATION; LETHAL RADIATION DOSE; MITOMYCIN; MUTAGENS; NITROUS ACID; POPULATION DYNAMICS; RADIOSENSITIVITY; SACCHAROMYCES CEREVISIAE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ULTRAVIOLET RADIATION; UPTAKE
- Descriptors DEC
- ANTIBIOTICS; ANTIMITOTIC DRUGS; DRUGS; ELECTROMAGNETIC RADIATION; ESTERS; FUNGI; HYDROGEN COMPOUNDS; INORGANIC ACIDS; ISOTOPE APPLICATIONS; MICROORGANISMS; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; PLANTS; RADIATION DOSES; RADIATIONS; SACCHAROMYCES; SULFONIC ACID ESTERS; YEASTS
Optional Information
- Notes
- 10 figs.; 1 tab.; 25 refs.; Updated automatically by Metadata and Full-Text Enrichment Agent