Use of HPLC for increasing the molar specific activities of tritium-labelled nucleosides

The possibility of chromotographic separation of different isotopic forms of tritiated nucleosides has been demonstrated. Using a reversed-phase column in 3-5% solutions of ethanol in water, the labelled nucleosides eluted ahead of corresponding unlabelled analogs. Such isotope effects allow simultaneous fine purification of nucleosides and its fractionation according to molar specific activity value. (Author)