Published January 14, 2008 | Version v1
Journal article Metadata-only

Ion irradiation in liquid of μm3 region for cell surgery

Creators

  • 1. Skobeltsyn Institute of Nuclear Physics, Moscow State University, Vorob'evy Gory, Moscow 119991 (Russian Federation)
  • 2. Kochi University of Technology, Tosa-Yamada, Kami, Kochi 782-8502 (Japan)
  • 3. Advanced Development and Supporting Center, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
  • 4. Cellular Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
  • 5. Atomic Physics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)

Description

We present here a cell surgery scheme involving selective inactivation or disruption of cellular structures. Energetic ions are injected into a cell through a tapered glass capillary like a microinjection method. A slight but essential difference from microinjection is that a thin window is prepared at the outlet so that no liquid material can flow in or back through the outlet while still allowing energetic ions to penetrate into the cell. An ∼MeV He ion beam from such a capillary having 10 μm outlet diameter inactivated a selected volume (∼μm3) of fluorescent molecules located in a HeLa cell nucleus

Additional details

Identifiers

DOI
10.1063/1.2834695;

Publishing Information

Journal Title
Applied Physics Letters
Journal Volume
92
Journal Issue
2
Journal Page Range
p. 023509-023509.3
ISSN
0003-6951
CODEN
APPLAB

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
39038496
Subject category
S71: CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; BIOPHYSICS; CAPILLARIES; HELA CELLS; HELIUM IONS; INACTIVATION; ION BEAMS; LIQUIDS; SURGERY; TAIL IONS
Descriptors DEC
ANIMAL CELLS; BEAMS; BIOLOGICAL EFFECTS; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CHARGED PARTICLES; FLUIDS; IONS; MEDICINE; ORGANS; PHYSICS; RADIATION EFFECTS; TUMOR CELLS

Optional Information

Notes
(c) 2008 American Institute of Physics