Published June 11, 2008 | Version v1
Journal article Metadata-only

Crystallization and crystallographic analysis of human NUDT16

Creators

  • 1. Graduate University, Chinese Academy of Sciences, Beijing 100049 (China)
  • 2. Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China)
  • 3. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100080 (China)
  • 4. Anhui Agricultural University, Anhui 230036 (China)
  • 5. Institute of Physics, Chinese Academy of Sciences, Beijing 100080 (China)

Description

Recombinant NUDT16 from human was expressed, purified and crystallized. The native crystals diffracted to 2.1 Å. Human NUDT16, a decapping enzyme belonging to the Nudix superfamily, plays a pivotal role in U8 snoRNA stability. Recombinant NUDT16 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals, which diffracted to 2.10 Å resolution, belonged to space group P212121, with unit-cell parameters a = 44.47, b = 79.32, c = 97.20 Å. The Matthews coefficient and the solvent content were calculated to be 1.92 Å3 Da−1 and 35.84%, respectively, for two molecules per asymmetric unit

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309108016928; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2443980

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2443980;
DOI
10.1107/S1744309108016928;
PII
S1744309108016928;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
64
Journal Issue
Pt 7
Journal Page Range
p. 639-640
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46065880
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; DIFFUSION; ESCHERICHIA COLI; MOLECULES; RESOLUTION; SOLVENTS; SPACE GROUPS; STABILITY
Descriptors DEC
BACTERIA; MICROORGANISMS; PHASE TRANSFORMATIONS; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2008
Notes
PMCID: PMC2443980; PMID: 18607096; PUBLISHER-ID: pu5223; OAI: oai:pubmedcentral.nih.gov:2443980