Published November 1978
| Version v1
Journal article
Investigation of the use of H2150 to estimate lipolysis and acyl-CoA hydrolysis
- 1. Wisconsin Univ., Madison (USA). Dept. of Radiology
Description
The possibility of measuring the rate of lipolysis by incorporation of 150 into fatty acids from H2150 has been investigated. It was found that there was a large incorporation of radioactivity from H2150 into chloroform-soluble material, even when lipolysis could not occur, especially with isolated mitochondria. The limits on sensitivity were found to be; the amount of H2150 used, the time taken from start of incubation to counting, and the amount of water incorporated into fatty acid. The usefulness of this isotope as a tracer in this type of work is discussed. (U.K.)
Additional details
Publishing Information
- Journal Title
- Int. J. Appl. Radiat. Isot.
- Journal Volume
- 29
- Journal Issue
- 11
- Journal Series
- Int. J. Appl. Radiat. Isot.
- Journal Page Range
- 694-696
- ISSN
- 0020-708X
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- United Kingdom
- INIS RN
- 10448730
- Subject category
- APPLIED LIFE SCIENCES; RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Numerical Data
- Quality check status
- Yes
- Descriptors DEI
- CHLOROFORM; EXPERIMENTAL DATA; HYDROLYSIS; LIPIDS; LIVER; METABOLISM; MITOCHONDRIA; OCTANOIC ACID; OXYGEN 15; RATS; SENSITIVITY; TABLES; TRACER TECHNIQUES; WATER;
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CHEMICAL REACTIONS; DATA; DATA FORMS; DECOMPOSITION; DIGESTIVE SYSTEM; EVEN-ODD NUCLEI; GLANDS; HYDROGEN COMPOUNDS; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MINUTES LIVING RADIOISOTOPES; MONOCARBOXYLIC ACIDS; NUCLEI; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANOIDS; ORGANS; OXYGEN COMPOUNDS; OXYGEN ISOTOPES; RADIOISOTOPES; RODENTS; SOLVOLYSIS; VERTEBRATES;