Protein transfer to nitrocellulose filters

The authors report that under their conditions the transfer efficiency of a given protein from a SDS-polyacrylamide gel to nitrocellulose filters depends on its Msub(r)-value. After immunodetection with specific antisera and 125I-labelled protein A, the amount of label bound to each antigen increases linearly with the amount of protein applied to the gel. The 125I-labelled protein A bound to the filter is not removed during successive incubations with different antisera. (Auth.)