Published June 26, 2019 | Version v1
Journal article Metadata-only

Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

Creators

  • 1. Department of Bioengineering, Imperial College London, London SW7 2AZ (United Kingdom)
  • 2. Helmholtz Pioneer Campus (HPC), Helmholtz Zentrum München, 85764 Neuherberg (Germany)
  • 3. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA (United States)
  • 4. Edwin L. Steele Laboratory for Tumour Biology, Massachusetts General Hospital, Boston, MA (United States)
  • 5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA (United States)

Description

The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 µm in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications. (paper)

Availability note (English)

Available from http://dx.doi.org/10.1088/1361-6463/ab16b4

Additional details

Identifiers

DOI
10.1088/1361-6463/ab16b4;

Publishing Information

Journal Title
Journal of Physics. D, Applied Physics
Journal Volume
52
Journal Issue
26
Journal Page Range
[6 p.]
ISSN
0022-3727
CODEN
JPAPBE

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
52051961
Subject category
S71: CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS;
Descriptors DEI
ANIMAL TISSUES; BRAIN; EMISSION; EXCITATION; FOCUSING; MICROSCOPY; MULTI-PHOTON PROCESSES; PENETRATION DEPTH; WAVELENGTHS
Descriptors DEC
BODY; CENTRAL NERVOUS SYSTEM; ENERGY-LEVEL TRANSITIONS; NERVOUS SYSTEM; ORGANS