Study on the biomass and size spectra of bio-particles in vermifilter biofilms

Highlights: • Large organisms increased while small ones decreased by the presence of earthworms. • Good linear models are found between abundance and size of bio-particles. • The VF has a higher productivity level of large organisms than the BF. • Temperature and humidity affect the VF performance. In biological processes of sludge treatment, the sludge yield is closely related to the energy dissipation of entire microbial system. The vermifilter (VF), a novel biofilter, works efficiently due to the introduction of earthworms, which modifies the energy flow pathway through the variations of microbial size structure. For a deep insight into the sludge reduction in the VF, the biomass size spectrum (BSS) was employed to map the energy dissipation in the VF. The results indicated that bio-particles in the size class of [31, 63] μm were reduced most in the excess sludge after the VF treatment. In biofilms, bio-particles in the size class of [31, 63] μm varied most with the filter depth and earthworm density. Eight biomass and size spectra (BSS) were established for all beds of the VF and BF (the control of the VF, without earthworms). The normalized BSS were all linear both in the VF and BF, and their linear regression parameters, the slopes (k) and intercepts (b), varied with the filter depth and the earthworm density. The k and b of the VF were both significantly different from those of the BF. According to the k, the productivity level of largest bio-particles was higher in the VF than in the BF. According to the b, bio-particles at the bottom of size structure could be taken faster in the VF than in the BF. At last, some improvement approaches with some tries were proposed to enhance the sludge treatment capacity of the VF.