Published October 1979 | Version v1
Journal article

Detection of hypoxanthine, xanthine and uric acid in γ-irradiated aqueous solution of cytosine

  • 1. Nihon Univ., Tokyo. Coll. of Agriculture and Veterinary Medicine

Description

The aqueous solution of cytosine of 3.6 x 10-2 M was irradiated with gamma -ray (60 megarad) in nitrogen-saturated glass ampules, and freeze-dried, then the residue obtained was changed to trimethylsilylacid, and this was analyzed by paper chromatography, UV spectrometry, and/or gas-liquid chromatography. Hypoxanthine, xanthine and uric acid were detected in this solution, in addition to some other compounds already known to be produced by gamma -irradiation, e.g., TMS-uracil, TMS-6-hydroxyuracil and TMS-hypoxanthine. It was presumed that these compounds were formed by the recombination of the primary radiolytic products. Uric acid formation by this mechanism was confirmed by gamma -irradiation of the mixture that contained urea, and 5- and 6-hydroxyuracil. (Kaihara, S.)

Additional details

Publishing Information

Journal Title
Nippon Nogeikagaku Kaishi
Journal Volume
53
Journal Issue
10
Series
Nippon Nogeikagaku Kaishi.
Journal Page Range
355-357
ISSN
0002-1407