In vivo and in vitro studies of the cyclophosphamide effect in radiopharmaceutical binding labelled with 99m Tc for the blood elements

The aim of this investigation is to study if cyclophosphamide (CP), in vivo or in vitro, is capable to interfere with the binding of 99m Tc O4- on blood elements. In in vivo results the % rad (% radioactivity) in P (plasma) C (cells), were not modified (in IF-P (insoluble fractions-plasma)) and SF-P (soluble fractions-plasma) were not altered by CP treatment. In IF-C (insoluble fractions-cells) the drug was able to increase the % rad. In in vitro results, the % rad in P and in C were not modified (in IF-P and SF-P until 6 h and IF-C and SF-C until 1 h). The differences between the effect of the CP in vivo and in vitro studies could be explained by the metabolism of this drug. (author). 23 refs, 4 tabs