Published November 2007 | Version v1
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Chapter 5. 99mTc-Hynic-Anx13: Preparation, stability, biodistribution and imaging of apoptosis

Creators

  • 1. Isotope Department, China Institute of Atomic Energy, Beijing (China)

Description

The paper describes an annexin V fragment modified with HYNIC (HYNICAnx13) and radiolabelled with 99mTc using tricine, EDDA or EDDA/tricine as coligands. The effects of various factors on 99mTc labelling were investigated. Chromatographic analysis, stability and biodistribution studies in mice and rats, and imaging of apoptosis in rats induced by cyclophosphamide, were performed. The labelled conjugates were stable in aqueous solution and in serum solution in vitro, but they were not stable in cysteine solution and in vivo. The imaging results were not satisfactory, indicating that HYNIC-Anx13 might not be a promising agent for apoptotic imaging. (author)

Part of:
Labelling of small biomolecules using novel technetium-99m cores

Additional details

Identifiers

URL
http://www-pub.iaea.org/MTCD/publications/PDF/TRS459_web.pdf; http://www-pub.iaea.org/MTCD/publications/publications.asp;

Publishing Information

Publisher
IAEA
Imprint Place
Vienna (Austria)
ISBN
92-0-101607-7
Imprint Title
Labelling of small biomolecules using novel technetium-99m cores
Imprint Pagination
335 p.
Journal Issue
no. 459
Series
Technical reports series
Journal Page Range
p. 89-101
ISSN
0074-1914

INIS

Country of Publication
Austria
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
39069779
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE; S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Descriptors DEI
APOPTOSIS; AQUEOUS SOLUTIONS; CHROMATOGRAPHY; CYSTEINE; ENDOXAN; IN VITRO; IN VIVO; LABELLING; MICE; RADIOISOTOPE SCANNING; RADIONUCLIDE KINETICS; RATS; STABILITY; TECHNETIUM 99
Descriptors DEC
ALKYLATING AGENTS; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; COUNTING TECHNIQUES; DISPERSIONS; DRUGS; HOMOGENEOUS MIXTURES; HOURS LIVING RADIOISOTOPES; IMMUNOSUPPRESSIVE DRUGS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; KINETICS; MAMMALS; MIXTURES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIOISOTOPES; RODENTS; SEPARATION PROCESSES; SOLUTIONS; TECHNETIUM ISOTOPES; THIOLS; VERTEBRATES; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
8 refs, 9 figs, 5 tabs
Secondary number(s)
STI/DOC--010-459