2,7-dichloro fluorescence: a suitable complexing agent for the spectrophotometric determination of ofloxacin in pure and pharmaceutical dosage forms

Ofloxacin is a broad spectrum antibiotic effective against many Gram positive and Gram negative bacteria as well as for some key anaerobes. Ofloxacin was reacted with 2,7- dichlorofluorescein in an acidified medium at room temperature to give a crimson-red complex which was measured at 430 to 460 nm (working wavelength 430 nm). thus providing a basis for a new spectrophotometric method of analysis for ofloxacin in pharmaceutical dosage forms. The complex in solution form obeyed the Beers Law between 0.02-0.25 mg/ml. The limit for detection was 0.02 mg/ml. The relative standard deviation was found to be 067 %. Similarly the molar absorptivity for complex was calculated to be 0.01 x 10/sup 4/ dm/sup 3/mole/sup -1/cm/sup -l/. This method was found to be useful for the estimation of ofloxacin in pure as well as in pharmaceutical formulations This method was easy to execute at laboratory level and required non cumbersome methodology with a relatively high precision and accuracy. (author)