Published April 14, 2014 | Version v1
Journal article Metadata-only

Self assembled monolayer based liquid crystal biosensor for free cholesterol detection

Creators

  • 1. Department of Science and Technology, Centre on Bimolecular Electronics, Biomedical Instrumentation Section, CSIR—National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110 012 (India)
  • 2. Polymeric and Soft Materials Section, CSIR—National Physical Laboratory (CSIR), Dr. K. S. Krishnan Road, New Delhi 110 012 (India)
  • 3. Centre for Physical and Mathematical Sciences, School of Basic and Applied Sciences, Central University of Punjab, City Campus, Mansa Road, Bathinda 151 001 (India)

Description

A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10 mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques

Additional details

Identifiers

DOI
10.1063/1.4871704;

Publishing Information

Journal Title
Applied Physics Letters
Journal Volume
104
Journal Issue
15
Journal Page Range
p. 154104-154104.5
ISSN
0003-6951
CODEN
APPLAB

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
45083853
Subject category
S46: INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY;
Descriptors DEI
AMMONIUM CHLORIDES; CHOLESTEROL; LIQUID CRYSTALS; SENSORS
Descriptors DEC
AMMONIUM COMPOUNDS; AMMONIUM HALIDES; CHLORIDES; CHLORINE COMPOUNDS; CRYSTALS; FLUIDS; HALIDES; HALOGEN COMPOUNDS; HYDROXY COMPOUNDS; LIQUIDS; ORGANIC COMPOUNDS; STEROIDS; STEROLS

Optional Information

Notes
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