Identification of functional group present in Andrographis paniculata (kalmegh) leaves by FTIR analysis

This present study aims to analyze the functional group present in the Andrographis paniculata leaves or also known as kalmegh by using Shimadzhu Fourier Transform Infrared (FTIR) with the absorbance range of 500 cm⁻¹ - 4000 cm⁻¹. Andrographis paniculata (Nees) is a valuable traditional medicinal plant and it has many important bioactive compounds. In this study, kalmegh leaves powder was soaked in two different solvent which were 80% aqueous methanol and distilled water. After soaked for 24 hours, the soaked kalmegh leaves was extracted using rotary evaporator to get the crude extract before being subjected to Fourier Transform Infrared (FTIR) spectroscopy for characterization of functional group contains in the leaves. Results obtained from this present research show six major compound group which are alkene, amine, aromatic, nitro, and alcohol. This six functional group were identified from various wavenumber of bending and stretching type of alkene group (894.97 cm⁻¹ and 1645.10 cm⁻¹), stretching type of amine (1080.11 cm⁻¹), stretching type of aromatic group (1409.96 cm ⁻¹), stretching type of nitro group (1543.05 cm⁻¹), stretching type of alcohol group (3323.35 cm⁻¹) and phenol group (3749.62 cm⁻¹). This study attempts to display the use of FTIR while simultaneously creating interest among related researchers in natural plant analysis. (paper)