Effects of NO on tumour cells radiosensitivity to γ-rays in vitro
- 1. Fudan Univ., Shanghai (China). Inst. of Radiation Medicine
- 2. Oncology Lab, the Pharmacy School of Manchest University (United Kingdom)
Description
To examine the effect of nitric oxide (NO) on tumour cells radiosensitivity, human sarcoma cells (HT1080) and its inducible nitrogen monoxide synthase (iNOS) gene transfected clones (iNOS9, iNOS12) were exposed to 1-5 Gy 60Co γ-ray irradiation alone or combined with 500 μmol/L L-NMMA, a NO synthesis inhibitor, in air or under hypoxia. It showed that both hypoxia and L-NMMA decreased the production of NO, but had no obvious effect on iNOS activity. With more NO formation, gene-transferred cells showed higher sensitivity to irradiation and lower OER than HT1080, but there was no obvious correlation between NO and cells sensitivity to irradiation. The results indicated that NO could take part in irradiation-induced cells damage and enhance cells radiosensitivity. (authors)
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 24
- Journal Issue
- 3
- Journal Page Range
- p. 171-174
- ISSN
- 1000-3436
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 38093660
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- AIR; ANAEROBIC CONDITIONS; ARGININE; CLONE CELLS; COBALT 60; GAMMA RADIATION; GENES; IRRADIATION; LIGASES; NITRIC OXIDE; OXYGEN ENHANCEMENT RATIO; RADIATION INJURIES; RADIOSENSITIVITY; SARCOMAS; TUMOR CELLS
- Descriptors DEC
- AMINO ACIDS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CARBOXYLIC ACIDS; CELL CULTURES; CHALCOGENIDES; COBALT ISOTOPES; DIMENSIONLESS NUMBERS; DISEASES; ELECTROMAGNETIC RADIATION; ENZYMES; FLUIDS; GASES; INJURIES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NEOPLASMS; NITROGEN COMPOUNDS; NITROGEN OXIDES; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SENSITIVITY; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 2 figs., 3 tabs., 8 refs.