Published May 1988 | Version v1
Journal article Metadata-only

Rapid and correct identification of intestinal Bacteroides spp. with chromosomal DNA probes by whole-cell dot blot hybridization

Creators

  • 1. Yakult Central Institute for Microbiological Research, Tokyo (Japan)

Description

A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these probes. Of 65 Bacteroides strains isolated from 19 human fecal specimens, which were identified as B. fragilis, B. thetaiotaomicron, B. ovatus, B. caccae, B. uniformis, B. stercoris, B. vulgatus, B. distasonis, and B. merdae by conventional phenotypic characterization, 62 (95%) were correctly identified with this hybridization procedure

Additional details

Publishing Information

Journal Title
Applied and Environmental Microbiology
Journal Volume
54
Journal Issue
5
Series
Appl. Environ. Microbiol.
Journal Page Range
1158-1162
ISSN
0099-2240
CODEN
AEMID

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
20005762
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACCURACY; BACTERIA; DNA; HYBRIDIZATION; INTESTINES; ORGANIZING; PHOSPHORUS 32
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; GASTROINTESTINAL TRACT; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOISOTOPES