The use of short-term and quasi-steady influx in estimating plasmalemma and tonoplast influx in barley root cells at various external and internal chloride concentrations

Chloride influx measured with 36Cl over a 10 min loading period followed by a 5 min wash in inactive solution is used as an estimate of influx across the plasmalemma in barley roots. Chloride influx measured over a 60 min loading period followed by a 30 min wash is used as an estimate of the quasi-steady influx from the external solution to the vacuole