Published May 1994 | Version v1
Journal article

Release of arachidonic acid from rat spleen lymphocytes after irradiation

  • 1. Kievskij Gosudarstvennyj Univ., Kiev (Ukraine)

Description

After spleen lymphocytes incubation with 14 C-arachidonic acid 14 C label was found associated predominantly with phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Addition of 0.5 mkM A23187 to the incubation media with 2mM Ca Cl2 caused a 4-fold increase in release of labeled free arachidonate in lymphocytes of the control animals. A dose-dependent increase in release of free arachidonate and membrane Ca2+ dependent phospholipase activity towards exogenous 2-14C-arachidonoyl-PE and -PC take place in a period 6-12 h after animal's irradiation in a doses 0.5 and 1.0 Gy. The maximum arachidonate release in 6 h following 1 Gy irradiation correlations with activation of more active phospholipase A2, hydrolising PE. Free arachidonate is supposed to be mobilized from different phospholipid pools, depending on the content of Ca2+ in the cells after irradiation

Additional details

Additional titles

Original title (Russian)
Высвобождение арахидоната из лимфоцитов селезенки крыс после облучения

Publishing Information

Journal Title
Ukrainskij Biokhimicheskij Zhurnal
Journal Volume
66
Journal Issue
3
Journal Page Range
p. 60-66.
ISSN
0201-8470
CODEN
UBZHD4