Comparison of transformation, DNA single strand breaks and its repair in human blood lymphocytes stimulated with PHA, ConA and PWM following exposure to γ-rays
Description
The transformation, DNA single strand breaks and its repair in human peripheral blood lymphocytes stimulated with PHA, ConA and PWM respectively following exposure to 60Co γ-rays were analyzed by 3H-thymidine incorporation and hydroxylapatite chromatography. The transformation of lymphocytes stimulated with PHA, ConA and PWM were suppressed by γ-rays and the dose-effect curves were biphasic within the range of 0-8 Gy. The lymphocytes stimulated with PWM were the most resistant to γ-rays. The extent of DNA single strand breaks in lymphocytes induced by γ-rays was linearly related to the dose within the range of 0-30 Gy and there was no marked difference in the three kinds of lymphocytes. After a period of post-irradiation (15 Gy) incubation at 37 deg C, the DNA single strand breaks could be rejoined but incompletely. The rejoined strands broke again if the cells were incubated longer. The repair ratio of single strand breaks in the lymphocytes stimulated with PWM was the highest among the cells with three mitogens. The results suggest that the difference of the radiation effect on transformation in different lytphocytes is probably related to the repair ability of DNA single strand breaks
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 7
- Journal Issue
- 4
- Series
- J. Radiat. Res. Radiat. Process.
- Journal Page Range
- 30-35
- ISSN
- 1000-3436
- CODEN
- FYYXE
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 22018832
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CELL DIVISION; CONCANAVALIN A; DNA; GAMMA RADIATION; IN VITRO; IRRADIATION; LABELLED COMPOUNDS; LYMPHOCYTES; LYMPHOKINES; PHYTOHEMAGGLUTININ; RADIOSENSITIVITY; STRAND BREAKS; THYMIDINE; TRACER TECHNIQUES; TRITIUM
- Descriptors DEC
- AGGLUTININS; ANIMAL CELLS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HEMAGGLUTININS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; IONIZING RADIATIONS; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; MATERIALS; MITOGENS; MUCOPROTEINS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYSACCHARIDES; PROTEINS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RIBOSIDES; SACCHARIDES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES