Published January 30, 1987 | Version v1
Journal article Metadata-only

Development and validation of an improved radioimmunoassay for serotonin in platelet-rich plasma

Creators

  • 1. Edinburgh Univ. (UK). Dept. of Medicine
  • 2. Western General Hospital, Edinburgh (UK)

Description

A radioimmunoassay (RIA) using a 125I-tracer is described for measurement of serotonin (5-hydroxytryptamine, 5-HT) in human platelet-rich plasma (PRP). Antisera were raised against 5-HT-succinamate conjugated to bovine albumin and, to improve assay sensitivity, the analyte was made chemically similar to the immunogen by conversion to N-acetylserotonin prior to assay, using the specific amino reagent N-acetoxysuccinimide. The assay shows good correlation with a high-pressure liquid chromatography (HPLC) reference method, indicating that no significant cross-reactions were detected. With this improved assay, it is possible to analyse up to 100 samples/day, compared with 10-20 samples/day by HPLC. (Auth.)

Additional details

Publishing Information

Journal Title
Clin. Chim. Acta
Journal Volume
162
Journal Issue
2
Series
Clin. Chim. Acta.
Journal Page Range
175-188
ISSN
0009-8981
CODEN
CCATA

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
18056864
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ACETYLATION; BLOOD PLASMA; BLOOD PLATELETS; COMPARATIVE EVALUATIONS; IODINE 125; LIQUID COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SEROTONIN
Descriptors DEC
ACYLATION; AMINES; AUTONOMIC NERVOUS SYSTEM AGENT; AZOLES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMICAL REACTIONS; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVALUATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRROLES; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS; SEPARATION PROCESSES; SYMPATHOMIMETICS; TRACER TECHNIQUES; TRYPTAMINES

Optional Information

Notes
28 refs.; 4 figs.; 2 tabs.