Studying the impact of ubiquitin on radiation-induced damage to genes through bioinformatic methodologies

Understanding the intricacies of cellular responses to radiation exposure is crucial for advancing radiobiology and developing effective strategies against radiation-induced pathologies. The annual radiation dose, sourced from natural and human-made sources, underscores the critical nature of this problem. Ubiquitination, as a pivotal process in protein regulation, holds the potential for alterations post-irradiation, impacting cellular recovery. The unique features of ubiquitin, beyond protein degradation, position it as one of the key players in comprehending cellular responses to radiation exposure. Our studies, employing bioinformatic methods, have revealed the potential use of ubiquitin in the context of radiation-induced cell exposure. We conducted an analysis to assess the commonality in the genetic spectrum between genes damaged by radiation and genes associated with ubiquitin. This study utilizes RNA sequencing to identify differentially expressed genes in mice subjected to irradiation. Our investigation delves into the impact of irradiation on ubiquitin-related genes, on various functions such as chromosome segregation and organelle fission. The heightened expression of genes associated with the cell cycle suggests the foundational role of ubiquitin-related genes in organism recovery after radiation exposure. The study not only contributes to advancements in radiobiology but also offers direction for developing preventive strategies against radiation-induced pathologies. (author)