Influence of rhTPO/GM-CSF fusion protein on hemopoiesis in mice irradiated with 60Co γ-rays
Creators
- 1. Institute of Radiation Medicine, Beijing (China)
Description
Objective: To find a new biological therapy for secondary hematopoietic failure including anemia, infection and hemorrhage after administration of chemotherapeutic drugs etc. Methods: hGM-CSF gene was ligated with hTPO gene isolated from human fetal liver mRNA and a new fusion protein rh TPO/GM-CSF obtained. Results: The new fusion protein could promote recovery of peripheral WBC and PLT of 5.0 Gy irradiated mice. BFU-E, CFU-Meg and CFU-GM in bone marrow of mice after irradiation recovered significantly by treatment with rhTPO/GM-CSF fusion protein for 10 days. Conclusion: These results suggest that the new fusion protein has the biological activity of both hTPO and hGM-CSF simultaneously and can stimulate the proliferation of megakaryocytes and granulocyte progenitors
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 19
- Journal Issue
- 4
- Journal Page Range
- p. 251-253
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 31007320
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANEMIAS; BIOLOGICAL FUNCTIONS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BLOOD FORMATION; BLOOD PLATELETS; CELL PROLIFERATION; CHEMOTHERAPY; COBALT 60; GAMMA RADIATION; HEMORRHAGE; LEUKOCYTES; MICE; PROTEINS
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; COBALT ISOTOPES; DISEASES; ELECTROMAGNETIC RADIATION; HEMIC DISEASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MATERIALS; MEDICINE; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PATHOLOGICAL CHANGES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RODENTS; SYMPTOMS; THERAPY; VERTEBRATES; YEARS LIVING RADIOISOTOPES