Oxidative coupling of Amoxicillin using 4-Aminoantipyrine: Stability and higher sensitivity

Amoxicillin (AMX) is a semi-synthetic β-lactam antibiotic belonging to the group of penicillins. When ingested, it is partially (about 80–90%) excreted as chemically unchanged via faeces and urine into the domestic sewage and thence discharged to the wastewater treatment plants, which subsequently, removing this substance from the aqueous solution is important. Therefore, the aim of current study was to determine the effects of different optimum conditions of the oxidative coupling calorimetric methods for the quantitative determinations of Amoxicillin. Methods: Amoxicillin was determined via using colorimetric spectrophotometric method in the alkali medium and adding 4-aminoantipyrine to give a panic-colored chromophore which is spectrophotometrically estimated at 509 nm, at room temperature in the presence of ferricyanide ion. Beer's law is observed in a concentration range 5-100 mgL⁻¹ and linearity(r²=0.9994) according to the proposed conditions. Results: The best concentration of 4AAP was 0.3gm/100 ml and that of the oxidant was 0.3gm/100 ml at the same order. Conclusion: the best results of reagent 4AAP is 0.3g/ml to give high stability indeed highly more absorptivity to enhance measurements and reduce error. (paper)