Crystallization and preliminary X-ray diffraction analysis of the full-length Mg²⁺ transporter MgtE

Selenomethionine-substituted crystals of the Mg²⁺ transporter MgtE from T. thermophilus diffracted to 3.5 Å resolution and belonged to space group C222₁, with unit-cell parameters a = 118.3, b = 134.9, c = 366.2 Å. The MgtE family of Mg²⁺ transporters are ubiquitously conserved in all three domains. The genes encoding full-length MgtE from seven different species were cloned. Three of the seven MgtE transporters were overexpressed and purified for use in crystallization trials. Only Thermus thermophilus MgtE was successfully crystallized using the sitting-drop vapour-diffusion method. Selenomethionine-substituted (SeMet) crystals were obtained by cross-microseeding using the native microcrystals. The SeMet crystals diffracted X-rays to 3.5 Å resolution using synchrotron radiation and belong to space group C222₁, with unit-cell parameters a = 118.3, b = 134.9, c = 366.2 Å. Structure determination is in progress