Cytotoxicity and genotoxicity of lanthanides for Vicia faba L. are mediated by their chemical speciation in different exposure media

Highlights: • Lanthanide toxic effects are not expected when phosphorous is present in media. • In test solution without P, LN showed genotoxicity effects in V. faba roots. • Chemical speciation calculations are needed to predict realistic LN toxic effects. • LN has high affinity to complex with PO₄, sulphates and carbonates. A comprehensive study of the toxicity of lanthanides (LN) in relation to the media composition will enhance the prediction of their potential adverse effects for living organisms. Here we examined the effect of different media on the V. faba root elongation and on the cytotoxic (mitotic index) and the genotoxic (micronucleated cell number) effects from toxicity tests with Ce, Gd and Lu (100, 800 and 6400 μg L⁻¹). Three different exposure media were selected: the standard Hoagland media (SH); an alternative SH, without phosphates (SH-P); and distilled water (DW). In the SH no cyto-genotoxic effects were observed and even, for low LN content, potential root elongation stimulation was reported. The absence of toxic effects was explained by a drastic decrease of the total dissolved LN concentration due to the presence of phosphates causing LN precipitation. In SH-P, LN remained largely soluble and inhibition of root elongation was observed mainly for the highest treatments. While in the tests done in DW, toxic effects were obtained for all treatments. Our results showed that in absence of phosphorous, LN appear mainly as free form and complexed in carbonates and sulphates, and can cause toxic effects, whereas toxicity is not expected when phosphorous is available in aquatic media. The highest LN root contents were observed for the tests using distilled water, possibly due to the absence of competition by Ca²⁺ for uptake. The present work demonstrated that media composition has a great impact in assessing the ecotoxicology of lanthanides.