Time effectiveness of DNA injury and its repair induced by irradiation using single cell gel electrophoresis

Objective: To improve the accuracy of estimation of radiation biodosimetry after radiation accident, to explore the time-effect relationship of DNA repair after irradiation, and to describe the curves and plane of DNA repair. Methods: The neutral single cell gel electrophoresis (SCGE) was performed to estimate the repair of DNA double-strand break (DNA-DSB) in human lymphocytes induced by different dose of radiation at 3, 24, 48, 72 h post irradiation. Results: Better goodness of fit was obtained not only in the dose-response curves at different time points, but also in the time-response curves at different doses. A smooth three-dimensional plane model was obtained when combining the two curves. Conclusions: The curves of DNA-DSB repair showed a linear relationship, and repair of radiation-plane model could be used for biological dosimetry. (authors)