Published April 2002
| Version v1
Journal article
Establishment of a method for RIA of transferrin
Creators
- 1. Shanghai Inst. of Radioimmunological Technology (China)
Description
Objective: To develop a method for RIA of transferrin without dilution of the serum sample. Methods: The measurable range was broadened through: Lowering of the specific radioactivity of the labelled antigen, increasing the concentration of the anti-serum and improve the curve fitting method. Results: The antiserum affinity was 3.82 x 10-10 mol/L, the specific radioactivity of the labelled antigen was 15.3 mg/mic, inter-assay error CV 5.38%, intra-assay error CV = 3.5% and the shape of the curve was good. For 30 normal control specimens, the mean TF content was 2.81 +- 0.972 mg/ml. Conclusion: Development of a method for RIA of TF without previous dilution of the test serum was of clinical value
Additional details
Publishing Information
- Journal Title
- Journal of Radioimmunology
- Journal Volume
- 15
- Journal Issue
- 2
- Journal Page Range
- p. 75-76
- ISSN
- 1008-9810
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 34032732
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANEMIAS; ANTIGENS; CHEMICAL PREPARATION; CONCENTRATION RATIO; DIAGNOSTIC TECHNIQUES; DOSE-RESPONSE RELATIONSHIPS; ERRORS; IMMUNE SERUMS; IODINE 125; KIDNEYS; LABELLING; LIVER; RADIOIMMUNOASSAY; TRANSFERRIN
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOASSAY; BODY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; GLOBULINS; GLOBULINS-BETA; HEMIC DISEASES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; METALLOPROTEINS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; SYMPTOMS; SYNTHESIS; TRACER TECHNIQUES