A new procedure for leukocytes labeling

Abscess localization using leukocytes labeled with In-oxine-111 is an established procedure. However, In-111 offers one half life (67 hr.) to the clinician to work with and is an expensive isotope. As such, a new approach offering versatility to the clinician in the manipulation of isotopic half life by substituting In-111 with isotopes of iodine I-123 (13 hr.) and I-131 (8 days) is studied. Oxine having an activated aromatic moiety was radioiodinated via Hunter-Greenwood method using I-125, yield being 90%. Therefore, judging from the in vitro tests, leukocytes stay viable after being labeled with radioiodinated oxine. Since isotopes by definition have similar chemical properties, for in vivo studies, I-125 (30-35 kev) could be substituted with I-123 (159 kev) and I-131 (364 kev)