Published October 1988
| Version v1
Journal article
Tritium isotope effect in high-pressure liquid chromatography of vitamin D metabolites
Description
A significant chromatographic isotope effect is reported for 1,25-dihydroxyvitamin D3 in a wide variety of HPLC separation systems. The effect is also observed for 24,25-dihydroxyvitamin D3. Retention times differ from less than 1% up to 4% depending on the separation system and the degree and position of tritium substitution. Such an effect must be corrected for whenever both labeled and unlabeled vitamin D metabolites are used in HPLC cochromatography or assay recovery studies
Additional details
Publishing Information
- Journal Title
- Analytical Biochemistry
- Journal Volume
- 174
- Journal Issue
- 1
- Series
- Anal. Biochem.
- Journal Page Range
- 137-141
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 20048646
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ISOTOPE EFFECTS; LIQUID COLUMN CHROMATOGRAPHY; METABOLISM; METABOLITES; TRACER TECHNIQUES; TRITIUM COMPOUNDS; VITAMIN D
- Descriptors DEC
- CHROMATOGRAPHY; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; SEPARATION PROCESSES; VITAMINS