Studies of isolation and purification of Acidithiobacillus ferrooxidans as well as their variabilities of growth characteristics

To explore plate separation techniques of Acidithiobacillus ferrooxidans and understand its growth characteristic, strains of Acidithiobacillus ferrooxidans were obtained by separation and purification using 2 : 2 solid medium. And growth characteristics of 5 strains of them were studied. The results showed that 2 : 2 solid medium could be used ideally for separation and purification of Acidithiobacillus ferrooxidans. And the fastest growth rate of Acidithiobacillus ferrooxidans could get at the initial pH value from 2.O to 2.5, the optimal growth temperature was 30 ℃ 9 the initial Fe²⁺ concentration might be desirable for 4.623 g/L, and the appropriate inoculums' quantity was 10%. There existed growth characteristics differences among different Acidithiobacillus ferrooxidans strains. (authors)