Bimetallic Au-Pd nanoparticles/RGO as theranostic nanoplatform for photothermal therapy of throat cancer - an in vitro approach

The current work showed the fabrication of bimetallic Au-Pd-NPs on reduced graphene oxide (RGO) sheets functionalized with plant polyphenols of Ziziphus ziziphus leaf extract for enhanced aqueous dispersibility using a simple green method. The synthesized Au-Pd/RGO nano composite was effectively employed for the in-vitro photothermal killing of HNE-1 cells. Applying the 808 nm laser (1 W cm^-2) and maintaining the concentration of Au-Pd/RGO at 75 μg mL^-1, the solution temperature was brought to 58 degree C, which is adequate for the eradication of cancer cells. Later, the solution temperature was brought to 80 degree C by increasing the concentrations to 90 μg mL^-1. Ultimately, the near total photo ablation (∼97%) of HNE-1 cells was obtained by maintaining the concentration of Au-Pd-RGO nano composites at ∼20 μg mL^-1, a laser power of 1 W cm^-1 and a LC50 value of 7μg mL^-1. This exceptional efficiency was attributed mostly to the synergistic effect of Au-Pd NPs, and RGO. The current study might lead the way for the synthesis of advanced materials that could be used as prospective photothermal candidates for the ablation of various unregulated cells