Published July 1983 | Version v1
Journal article Metadata-only

Separation of human lymphocytes on Ficoll-Paque gradients: stimulation of cells and depletion of a concanavalin-A responsive radioresistant subpopulation

Creators

  • 1. Ben Gurion Univ. of the Negev, Beer Sheva, Israel

Description

A subpopulation of human lymphocytes separated on Ficoll-Paque gradients showed an ultrastructural phenotype characteristic of stimulated cells. Thymidine incorporation was increased fivefold compared with unseparated (Buffy coat) controls. The Ficoll-Paque separated lymphocytes were more sensitive to γ radiation than unseparated lymphocytes and showed a decreased capability to undergo transformation in response to concanavalin A. Transformation in response to phytohemagglutinin and pokeweed mitogen was the same as for unseparated control lymphocytes. These results are interpreted as the selective depletion of a Con A-responsive T-cell fraction by Ficoll-Paque separation

Additional details

Publishing Information

Journal Title
Radiat. Res.
Journal Volume
95
Journal Issue
1
Series
Radiat. Res.
Journal Page Range
108-115
ISSN
0033-7587

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
15001642
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
CENTRIFUGATION; COBALT 60; CONCANAVALIN A; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; LYMPHOCYTES; RADIOSENSITIVITY; STIMULATION; ULTRASTRUCTURAL CHANGES
Descriptors DEC
AGGLUTININS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HEMAGGLUTININS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; MATERIALS; MINUTES LIVING RADIOISOTOPES; MORPHOLOGICAL CHANGES; NUCLEI; ODD-ODD NUCLEI; RADIATIONS; RADIOISOTOPES; SEPARATION PROCESSES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES