Published July 2015 | Version v1
Journal article Metadata-only

Impedimetric aptamer-based determination of the mold toxin fumonisin B1

Creators

  • 1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, 214122 (China)

Description

We are presenting an aptasensor for the sensitive determination of fumonisin B1 (FB-1) via electrochemical impedance spectroscopy (EIS) and applying aptamer-based biorecognition. A thiolated aptamer for FB-1 was anchored onto the surface of gold nanoparticles (AuNPs) on a glassy carbon electrode. A significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100 μM concentration range, and the detection limit is as low as 2 pM. The assay was applied to determine FB-1 in spiked maize samples and gave recovery rates ranging from 91 to 105 %. The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysis. (author)

Additional details

Identifiers

DOI
10.1007/s00604-015-1492-x;

Publishing Information

Journal Title
Microchimica Acta (Online)
Journal Volume
182
Journal Issue
9-10
Journal Page Range
p. 1709-1714
ISSN
1436-5073

INIS

Country of Publication
Austria
Country of Input or Organization
Austria
INIS RN
47046141
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
CARBON; DNA; ELECTROCHEMISTRY; ELECTRODEPOSITION; ELECTRODES; FUNGI; GOLD; MAIZE; NANOCHEMISTRY; NANOPARTICLES; SENSITIVITY; SURFACES; THIOLS; TOXICITY; TOXINS
Descriptors DEC
ANTIGENS; CEREALS; CHEMISTRY; DEPOSITION; ELECTROLYSIS; ELEMENTS; GRAMINEAE; HAZARDOUS MATERIALS; LILIOPSIDA; LYSIS; MAGNOLIOPHYTA; MATERIALS; METALS; NONMETALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARTICLES; PLANTS; SURFACE COATING; TOXIC MATERIALS; TRANSITION ELEMENTS