Published July 1987
| Version v1
Journal article
Rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides
Description
An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles
Additional details
Publishing Information
- Journal Title
- J. Lipid Res.
- Journal Volume
- 28
- Journal Issue
- 7
- Series
- J. Lipid Res.
- Journal Page Range
- 873-877
- ISSN
- 0022-2275
- CODEN
- JLPRA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 19017798
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ACCURACY; CARBOXYLIC ACIDS; CELL CULTURES; MACROPHAGES; MICE; NICKEL 63; RADIOENZYMATIC ASSAY; SENSITIVITY ANALYSIS; TRACER TECHNIQUES; TRIGLYCERIDES; TRIOLEIN
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CONNECTIVE TISSUE CELLS; ESTERS; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIPIDS; MAMMALS; NICKEL ISOTOPES; NUCLEI; OILS; ORGANIC ACIDS; ORGANIC COMPOUNDS; OTHER ORGANIC COMPOUNDS; PHAGOCYTES; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES; YEARS LIVING RADIOISOTOPES