Effects of 60Co γ-rays on PWM and LPS induced lymphocytes

The relationship between lymphocytes induced by PWM and LPS was investigated by means of 3H-TdR and 14C-UR incorporation. The study showed that in votro, PWM-induced cells were able to promote the stimulating effect of LPS to B lymphocyte. The stimulating effect of PWM-induced cells was obviously weakened after PWM cells being irradiated with γ-rays. When PWM-induced cells and LPS-induced cells were incubated together, with one kind of cells exposed to 60Co γ-rays, incorporation value of 3H-TdR became much smaller and the synergetic function disappeared, especially, when PWM-induced cells were irradiated. For patients suffering from carcinoma of nasopharynx, when treated with 60Co γ-rays, the incorporation value in LPS-induced cells approached normal level, while the incorporation value in PWM-induced cells reduced significantly and the stimulating effect of PWM-induced cells on LPS-induced cells became much weaker. The fact described above demonstrated that PWM-induced cells have the function of T-helper cells and play a more important role in the synergy than LPS induced cells