Ca uptake and its influence by growth hormone in osteoblasts of fetal rat calvaria

Uptake and release of Ca2+ are important functions of osteoblasts. In the paper we studied the uptake of calcium and influence by Growth Hormone in osteoblasts of fetal rat calvaria by liquid scintillation spectrometry of 45Ca2+. In short-term cultures of the bone derived cells, the uptake of 45Ca2+ increased steadily. The activity of 45Ca2+ in the cells of 15 minute cultures was 2∼3 times of that in the 0 minute cultures. It continued to increase in the cells of 30 minute cultures. Exposure of the bone cells to GH at 55.3 ng/ml increased the uptake of 45Ca2+ by 2.3 times in the 30 minute cultures and 1.5 times in the 60 minute cultures than those of the control