Effects of ⁶⁰Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro

Objective: To investigate the effects of ⁶⁰Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro. Methods: The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to ⁶⁰Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃ ,and then mixed with the unirradiated blood samples of the Microscopy was used to observe the chromosome aberration double (centromere + centromere) and the biological dose was estimated thereby. Results: The amounts of double centromere + centromere were increased along with the dose of irradiation in all groups. The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy, and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy. Conclusion: Chromosome aberration can be used as a biomarker in estimation of uneven irradiation. (authors)