Studies on the application of microorganisms immobilized by radiation in the pharmaceutical industry

Immobilization of the bacteria A. simplex by-2-13 was obtained by irradiation polymerization with an electron beam and gamma radiation at 0 deg C. Prednisone acetate was derived from cortisone acetate by 1,2-dehydrogenation, catalyzed by immobilized A. simplex by-2-13. Scanning electron micrographs documented the structure of the immobilized bacteria. In this work, the effect of absorbable doses of radiation and the reaction time of non-immobilized bacteria on dehydrogenase activity were studied. The effect of pH, temperature and concentration of substrate were also studied. (author) 17 refs.; 6 figs.; 1 tab