Effects of benzopyrene on the capacity of antioxidases, non-specific immunity and organization structure in liver and kidney of carp( Cyprinus carpio)

To investigate the toxic mechanism of BaP on carp (Cyprinus carpio), the antioxidases (SOD, CAT, GSH) activities, MDA content, and the activities of non-specific immunity enzymes (LZM, PO, AKP, ACP), NBT positive cells and organization structure in liver and kidney of carp exposed to different concentrations of BaP (0, 0.1, 0.5, 1.0 μg · L^-1) for 30 days were studied. The results showed that after exposure for 30 days, SOD activity and MDA content in liver and kidney, CAT activity in liver, and GSH activity in kidney exposed to 1.0 μg · L^-1 of BaP were significantly lower than those in the control group. The activities of LZM, AKP and PO in liver and kidney, and ACP activity in liver exposed to 1.0 μg · L^-1 of BaP were significantly decreased compared with control group. The number of NBT positive cells decreased significantly compared with control group. The liver and kidney tissue were damaged to different degrees. Correlation analysis indicated that the BaP concentration was negatively correlated with SOD activities, was positively correlated with MDA content in liver and kidney, had a negative correlation with the activities of AKP, ACP, PO in liver and kidney, was negatively correlated with the number of NBT positive cells. All above indicated that exposure to BaP in water could negatively affect the antioxidant ability, non-specific immunity function and organization structure in liver and kidney of carp to some extent, which provided the important information for further study of the ecological toxicity of BaP. (authors)