Effects of predator movement patterns on the biofouling layer during gravity-driven membrane filtration in treating surface water

Highlights: • Predator movement patterns at different temperatures were investigated. • Intense activity of predators was favorable to the stable flux. • The size and shape of spongy flocs grazed by predators were important to the system. • Regulating the biofouling layer could improve gravity-driven membrane performance. • A linear correlation was observed between the stable flux and the temperature. Biological predation has a significant effect on biofouling layers in gravity-driven membrane (GDM) filtration systems. However, the detailed process of predatory activities is still not well known. This study explored the effects of predator movement patterns on the biofouling layer at different temperatures and the factors affecting the stable flux level. The results indicated that Demospongiae, Spirotrichea and Saccharomycetes were the main species, with the body contracting or rotating in one position at 5 °C, and Litostomatea accounted for 55.1% at 10 °C. The weak agility of these species resulted in a less porous biofouling layer with a high extracellular polymeric substance (EPS) concentration, which was responsible for the low permeate flux and the time to reach flux stability. Bdelloidea was dominant at 20 and 30 °C, and the more heterogeneous biofouling layer with a lower EPS concentration was related to their intense creeping and swimming movements and their ability to create current in the water. The grazing of spongy flocs by predators affected the GDM system performance, and a high stable flux was obtained with large and loose flocs. In addition, the diversity of the eukaryotic community decreased after the flux stabilized due to the particular predominance of Bdelloidea at high temperatures, corresponding to a high stable flux. Pollutant removal was less affected by eukaryotes, and decreased ammonia nitrogen removal rates were related to the lower activity of nitrifying bacteria. Moreover, the reliable linear correlation between the temperature and the stable flux implied that the stable flux could be well predicted in the GDM system. The findings are beneficial for developing new strategies for regulating flocs and the biofouling layer to improve the performance of GDM systems.