Published February 1997
| Version v1
Journal article
The enzymatic preparation of [α-32P] ATP with high specific activity
Creators
- 1. Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy
Description
A modified procedure for preparing [α-32P]ATP is described with which [γ-32P]GTP is used as phosphate donor instead of [γ-32P]ATP. The specific activity of the resulted [α-32P]ATP in other experiments is raised greatly as to 148 PBq/mol or more, without decreasing the yield of [α-32P]ATP which is over 93% based on 32Pi. The specific activity is measured by HPLC and the factors that affect the specific activity of [α-32P]ATP are discussed
Additional details
Publishing Information
- Journal Title
- Journal of Nuclear and Radiochemistry
- Journal Volume
- 19
- Journal Issue
- 1
- Journal Page Range
- p. 46-50.
- ISSN
- 0253-9950
- CODEN
- HHHHDH
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 28046422
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- ACTIVITY LEVELS; ATP; CHEMICAL PREPARATION; ENZYMES; LABELLING; LIQUID COLUMN CHROMATOGRAPHY; PHOSPHORUS 32
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; SEPARATION PROCESSES; SYNTHESIS