Influence of in vitro irradiation upon LIF production by ConA stimulated mononuclear cells
Description
Leukocyte migration inhibitory factor (LIF) activity of culture supernatants of in vitro irradiated Concanavalin A (ConA) stimulated lymphocytes was tested by measuring granulocyte migration from clotted plasma droplets placed in flat bottom microplates. The specificity of inhibition was assured by pretreating the assay supernatants with anti-LIF antibodies which abrogated granulocyte migration inhibition but did not impair guinea pig Peritoneal Exudate Cells (PEC) migration inhibition. In vitro irradiation (150-1200 rad) of MNC cultures either before or after ConA stimulation did not impair lymphokine production and sometimes significantly improved the supernatants' LIF activity as compared with that of unirradiated cultures. The existence of radiosensitive suppressor cells regulating LIF production by ConA stimulated mononuclear cells is suggested
Additional details
Publishing Information
- Journal Title
- Int. J. Radiat. Oncol., Biol. Phys.
- Journal Volume
- 7
- Journal Issue
- 12
- Series
- Int. J. Radiat. Oncol., Biol. Phys.
- Journal Page Range
- 1679-1683
- ISSN
- 0360-3016
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 14749695
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANTIBODIES; BIOLOGICAL RADIATION EFFECTS; CONCANAVALIN A; IN VITRO; INHIBITION; LYMPHOCYTES; LYMPHOKINES; MITOGENS; MONOCYTES; STIMULATION
- Descriptors DEC
- AGGLUTININS; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; HEMAGGLUTININS; LEUKOCYTES; MATERIALS; RADIATION EFFECTS; SOMATIC CELLS