Screening of the diversity mutational strains of converting D-xylose to ethanol by 60Co-γ irradiat ion and its fermentat ion characterizat ion reaserch
Creators
- 1. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilizat ion/Hunan Agricultural University, Changsha (China)
- 2. The Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences, Beijing (China)
- 3. College of Food Science and Technology, Hunan Agricultural University, Changsha (China)
- 4. Hunan Collaborat ive Innovation for Utilizat ion of Botanical Functional Ingredients, Changsha (China)
Description
In order to lucubrate the metabolic mechanism of D-xylose into ethanol by the method of Comparative genomics, we used 60Co-γ irradiation to mutagenize Pichia stipitis CICC1960 and obtained some high ethanol conversion strains and seven mutational strains. Using the ethanol yield as index, the fermentation ability of mutational strains in descending order was: a1, X4, i11, K2, X21, CICC1960, a11, c10, and the average of ethanol-xylose ratio respectively was: 0.3143, 0.3037, 0.2911, 0.2678, 0.2578, 0.2311, 0.2230, 0.1220. The strain a1, X4, i11 were dominant strains. The ethanol yield of strain a1 was 15.6 g·L-1 at 72 h, 140% higher than the parent strain, and its ethanol-xylose ratio 37.9% higher than the parent strain. The ethanol yield of strain X4 was 15.4 g·L-1 at 60h, 234.8% higher than the parent strain, and its ethanol-xylose ratio 33.1% higher than the parent strain. The ethanol yield of strain i11 was 14.8 g·L-1 at 60h, 221.7% higher than the parent strain, and its ethanol-xylose ratio 29% higher than the parent strain. (authors)
Additional details
Publishing Information
- Journal Title
- Journal of Nuclear Agricultural Sciences
- Journal Volume
- 29
- Journal Issue
- 2
- Journal Page Range
- p. 290-295
- ISSN
- 1000-8551
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 51032465
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- COBALT 60; ETHANOL; FERMENTATION; GAMMA RADIATION; IRRADIATION; MUTATIONS; RADIATION INDUCED MUTANTS; XYLOSE
- Descriptors DEC
- ALCOHOLS; ALDEHYDES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOCONVERSION; CARBOHYDRATES; COBALT ISOTOPES; ELECTROMAGNETIC RADIATION; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; MONOSACCHARIDES; MUTANTS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PENTOSES; RADIATIONS; RADIOISOTOPES; SACCHARIDES; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 6 figs., 30 refs.