Published 1976 | Version v1
Book Metadata-only

Adrenocorticotropic hormone (ACTH), ch. 7

Creators

  • 1. Massachusetts General Hospital, Boston (USA)
  • 2. National Inst. of Arthritis, Metabolism, and Digestive Diseases, Bethesda, Md. (USA)

Description

The method described for ACTH determination is based on the use of specific ACTH receptors from plasma membrane rich fractions of adrenal cortical tumor homogenates. This receptor is specific for biologically active ACTH. ACTH is labelled with 125I using a modification of the Hunter-Greenwood method. After iodination, the iodinated ACTH is separated from unlabelled ACTH over a column of carboxymethylcellulose, using an ammonium acetate gradien as eluant. The thus obtained 125I-ACTH with high specific activity has a biological activity that varies from 70% when fresh to 40% after 6 weeks. The assay is performed in siliconized glassware. After incubation, the bound and free hormones are separated by adsorption of 125I-ACTH in QUSO pellets

Additional details

Additional titles

Subtitle (English)
Radioreceptor assay, 7.2

Publishing Information

Publisher
North-Holland.
Imprint Place
Amsterdam, Netherlands
ISBN
0 7204 0630 7
Imprint Title
Methods in radioimmunoassay of peptide hormones
Imprint Pagination
p. 89-95.

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
8301293
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACTH; ADRENAL GLANDS; ADSORPTION; BIOCHEMISTRY; CHROMATOGRAPHY; HOMOGENATES; INCUBATION; IODINE 125; LABELLING; ORGANIC IODINE COMPOUNDS; RADIOACTIVITY; RADIOASSAY; SPECIFICITY; TUMOR CELLS
Descriptors DEC
ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BODY; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOISOTOPES; SEPARATION PROCESSES

Optional Information

Notes
Imprint:Reprinted from Methods in Investigative and Diagnostic Endocrinology, vols. 2A and 2B, 1973, North-Holland.