Gene and protein targeting technologies create novel opportunities in plant biotechnology

An important platform technology that is missing in the field of plant science is gene targeting through homologous recombination. Moreover, for many economically important plant varieties, robust and efficient protocols for vegetative propagation and genetic modification are still lacking. The availability of such technologies is essential for the further development of plant biotechnology. Add2X Biosciences BV is a young biotech spin-off from Leiden University that aims to create novel opportunities in plant biotechnology, by developing innovative platform technologies and products for the directed and efficient delivery of DNA and proteins into plant cells. The Add2X portfolio includes: 1. Efficient gene targeting in plants through suppression of the non-homologous recombination pathway. Proof of concept has been obtained in different yeasts and filamentous fungi. Currently, research is in progress to confirm the applicability of this technology in plant species. 2. Agrobacterium-mediated protein translocation to produce and transiently 'inject' proteins of interest into plant cells in order to exert their function without permanently altering the host cells. 3. Induction of somatic embryogenesis in hitherto recalcitrant crop species. A protein family was identified that, when over-expressed, stimulates the spontaneous formation of somatic embryos from vegetative plant cells. The Add2X technologies stand well on their own, but clearly have the potential to be combined into integrated technologies or products. The technologies are developed in close collaboration with Leiden University. In addition, Add2X has established strategic alliances with other academic and industrial partners to explore novel opportunities and to achieve rapid implementation of the technologies in the biotechnology sector. (author)