Development of cardiac troponin-I radioimmunoassay and preliminary results in clinical application
Creators
- 1. National Laboratory of Nuclear Medicine, Wuxi (China)
Description
A double antibody radioimmunoassay (RIA) was developed to measure cardiac troponin-I (CTnI) in serum. CTnI from fresh human myocardium was purified and identified. Antisera against CTnI were raised in NZ rabbits with affinity constant (Ka) 4.1 x 10-10 L/mol. Iodination of CTnI was carried out with iodogen. The specific activity of the product was 3659 GBq/g. The ED40 was 6.4 +- 0.34 μg/L (x-bar +- s, n = 8) from standard curves. The sensitivity was 1.5 μg/L and the assay covered the range 2∼64μg/L for CTnI. The intra-assay and inter-assay coefficient of variation (CV) were 2.1%∼5.7% (n = 8) and 5.4%∼9.9% (n = 4) respectively. The assay recovery was 90.1%∼95.3%. Non-immunochemical cross reactivity with skeletal troponin-I(STnI) was observed. The normal value was 2.17 +- 0.66 μg/L (x-bar +-s) in sera of 87 normal persons
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Nuclear Medicine
- Journal Volume
- 14
- Journal Issue
- 3
- Journal Page Range
- p. 164-166.
- ISSN
- 0253-9780
- CODEN
- CITCDE
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 26050319
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BLOOD; CALCIUM COMPLEXES; DIAGNOSIS; IODINE 125; LABELLED COMPOUNDS; MYOCARDIAL INFARCTION; MYOCARDIUM; PROTEINS; RADIOIMMUNOASSAY; SENSITIVITY
- Descriptors DEC
- ALKALINE EARTH METAL COMPLEXES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; COMPLEXES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HEART; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; MUSCLES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES