Mechanism of INF-γ antagonizing pulmonary fibroblast proliferation stimulated by 60Co γ-rays
- 1. Beijing Inst. of Radiation Medicine, Beijing (China)
Description
Objective: To explore the mechanism of IFN-γ antagonizing human pulmonary fibroblasts (HLF) proliferation stimulated by 60Co γ-rays. Methods: The time- and dose-effect of HLF proliferation promoted by TGF-β1 and rat serum after radiation as well as effect of IFN-γ on proliferation were observed by MTT colorimetric methods. Content of TGF-β1 in rat serum and pulmonary tissues after irradiation as well as the effect of IFN-γ on rat serum after radiation promoting HLF to synthesize TGF-β1 were determined by ELISA. Results: TGF-β1 played a role in promoting HLF proliferation. The sera of irradiated rats could promote HLF proliferation and TGF-β1 synthesis, but the effect was inhibited by IFN-γ significantly (P<0.05). The level of TGF-β1 in rat serum and lung tissues gradually increased from 1 to 4 weeks after irradiation. Conclusions: TGF-β1 is involved in the process of γ-rays stimulating HLF proliferation, while IFN-γ can inhibit HLF from synthesizing TGF-β1 and antagonize its role of promoting HLF proliferation. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 28
- Journal Issue
- 1
- Journal Page Range
- p. 13-16
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 41095357
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; BLOOD SERUM; CELL PROLIFERATION; COBALT 60; DOSES; ENZYME IMMUNOASSAY; FIBROBLASTS; GAMMA RADIATION; GROWTH FACTORS; HUMAN POPULATIONS; INHIBITION; IRRADIATION; LUNGS; RATS; SYNTHESIS
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOASSAY; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY; BODY FLUIDS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; MITOGENS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; POPULATIONS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RESPIRATORY SYSTEM; RODENTS; SOMATIC CELLS; VERTEBRATES; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 4 figs., 14 refs.