Published March 4, 2005 | Version v1
Journal article Metadata-only

Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films

Creators

  • 1. Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201 (United States)

Description

We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field

Additional details

Identifiers

DOI
10.1016/j.bbrc.2004.12.148;
PII
S0006-291X(04)02975-4;

Publishing Information

Journal Title
Biochemical and Biophysical Research Communications
Journal Volume
328
Journal Issue
1
Journal Page Range
p. 78-84
ISSN
0006-291X
CODEN
BBRCA9

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
36062902
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANISOTROPY; CROSS SECTIONS; DNA; EXCITATION; FLUORESCENCE; LASERS; PHOTONS; SAPPHIRE; SILVER
Descriptors DEC
BOSONS; CORUNDUM; ELEMENTARY PARTICLES; ELEMENTS; EMISSION; ENERGY-LEVEL TRANSITIONS; LUMINESCENCE; MASSLESS PARTICLES; METALS; MINERALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXIDE MINERALS; PHOTON EMISSION; TRANSITION ELEMENTS

Optional Information

Copyright
Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.