Published July 19, 2011 | Version v1
Journal article Metadata-only

Preliminary X-ray crystallographic studies of UDP-glucose-4-epimerase from Aspergillus nidulans

Creators

  • 1. University of Saskatchewan, 110 Science Place, Saskatoon, Saskatchewan S7N 5C9 (Canada)
  • 2. University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5E2 (Canada)

Description

UDP-glucose-4-epimerase from A. nidulans has been crystallized as a complex with the substrate UDP-galactose using the microbatch method under paraffin oil. UDP-glucose-4-epimerase (GALE) from Aspergillus nidulans was overexpressed in Escherichia coli, purified via His-tag affinity chromatography and cocrystallized with UDP-galactose using the microbatch method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation on the Canadian Light Source 08ID-1 beamline. Examination of the data with d*TREK revealed nonmerohedral twinning, from which a single lattice was ultimately extracted for processing. The final space group was found to be C2, with unit-cell parameters a = 66.13, b = 119.15, c = 161.42 Å, β = 98.48°. An initial structure solution has been obtained via molecular replacement employing human GALE as a template model

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309111020914; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151119

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151119;
DOI
10.1107/S1744309111020914;
PII
S1744309111020914;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
67
Journal Issue
Pt 8
Journal Page Range
p. 885-887
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46072904
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
AFFINITY; CRYSTALS; ESCHERICHIA COLI; GLUCOSE; LIGHT SOURCES; MATHEMATICAL SOLUTIONS; PARAFFIN; RESOLUTION; SOLUTIONS; SPACE GROUPS; SUBSTRATES; SYNCHROTRON RADIATION; TWINNING
Descriptors DEC
ALDEHYDES; ALKANES; BACTERIA; BREMSSTRAHLUNG; CARBOHYDRATES; DISPERSIONS; ELECTROMAGNETIC RADIATION; HEXOSES; HOMOGENEOUS MIXTURES; HYDROCARBONS; MICROORGANISMS; MIXTURES; MONOSACCHARIDES; ORGANIC COMPOUNDS; OTHER ORGANIC COMPOUNDS; RADIATION SOURCES; RADIATIONS; SACCHARIDES; SYMMETRY GROUPS; WAXES

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2011
Notes
PMCID: PMC3151119; PMID: 21821886; PUBLISHER-ID: hc5133; OAI: oai:pubmedcentral.nih.gov:3151119