In vitro and in vivo studies on red blood cell labelling using 111indium oxine, 111indium oxine-sulphate and sup(99m)Tc oxine

The optimal conditions for red blood cell labelling using 111indium oxine, 111indium oxine sulphate and sup(99m)Tc oxine were established both in vitro as well as in vivo. The use of either acid citrate dextrose or sodium citrate as anticoagulant had no effect on labelling efficiency. Other variables such as the incubation time, temperature, duration, cell number and concentration of the complex exert a significant influence on labelling efficiency. Labelling efficiency of red blood cells is very high also under non-optimum conditions as compared with other cells (leucocytes, platelets). (Author)